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STAB VIDA is strategically located in the research and technology park of Madan Parque - FCT / UNL, with administrative and financial facilities, fully equipped laboratories with ISO9001:2015 and ISO13485:2018 certifications and a team specialized with more than 20 years of experience in the areas of genetics and genomics using PCR and nucleic acid sequencing technologies.

STAB VIDA, created in 2001, is a Portuguese SME in the field of biotechnology made up of 30 employees and an exclusive line of genetics and genomics products and services. Currently, its commercial territory covers Portugal, Spain, the United Kingdom, Italy, South America (Brazil, Chile and Uruguay) and Africa (Angola and Nigeria). The STAB VIDA team is made up of a CEO, CFO, COO, Project Manager, laboratory and manufacturing technicians, researchers and a commercial team.

STAB VIDA also has extensive experience in participating and coordinating national and European projects (FP6, FP7 and H2020). Special mention should be made of the participation of STAB VIDA in the EuroGentest NoE FP6 project as it allowed the entire genetic testing process to be harmonized, from sampling to processing, across Europe, which will be extremely important for the implementation of the easierNGS project and coordination of the Pulmagene, LungCard FP7 and LungCARD RISE projects, specifically in the design of the lung cancer pharmacogenomics NGS panel.

STAB VIDA has ISO9001:2008 certified laboratories, fully equipped, and boasts a highly specialized team with several years of experience in genetics and genomics. Its primary focus on research and development, as well as commercialization, is centered around point-of-care diagnosis and theragnostics, especially for diseases such as Alzheimer's, Lyme, and Cancer. Additionally, the company is dedicated to oligonucleotide synthesis, bioinformatics, and nucleic acid sequencing, including Next-Generation Sequencing (NGS), crucial areas in the biotechnology, biomedical research, and clinical community sectors.

Key Infrastructures:

- Comprehensive facilities for nucleic acid extraction, amplification, analysis, and storage, equipped with a robotic pipetting platform, PCR and Real-Time thermocyclers, horizontal and vertical electrophoresis systems, gel doc equipment, centrifuges, freezers, among other molecular biology devices.

- Facilities dedicated to nucleic acid sequencing, including three gold-standard automated capillary sequencers based on Sanger technology (ABI PRISM 3130 and 3730 systems); an Ion Torrent NGS platform (Life Technologies); and various genetic analysis equipment, with licenses for CLC Genomics workbench and other bioinformatics tools. The company also has access to HiSeq 2500 (Illumina) and MiSeq (Illumina) platforms for comprehensive and effective validation of easierNGS on different NGS platforms.

Mechanisms of induction of chromosomal aberrations by hydroquinone in V79 cells

By Céu Silva M, Gaspar J, Duarte Silva I, Leão D, Ruef J

Hydroquinone occurs naturally in bacteria and plants and it is also manufactured for commercial use. Human exposure to this compound can occur by environmental, occupational, dietary and cigarette smoke exposure and from exposure to benzene, which can be metabolized to this compound. However, the main source of exposure to this compound is dietary, since hydroquinone is a naturally occurring compound in many foods.Hydroquinone can be metabolized to benzoquinones, which are potent haematotoxic, genotoxic and carcinogenic compounds that can also induce the formation of radical species, predisposing cells to oxidative damage. In order to clarify the involvement of radical species in the genotoxicity of hydroquinone, the induction of chromosomal aberrations in V79 cells was studied along with the assessment of the production of hydroxyl radicals at different pH values (6.0, 7.4 and 8.0), as well as the effect of antioxidant enzymes [catalase and superoxide dismutase (SOD)] on the clastogenic effect of hydroquinone. The results obtained indicate that the clastogenic activity of hydroquinone is dependent on the pH, suggesting that deprotonation is a fundamental step leading to DNA lesions under the experimental conditions used. The addition of S9 mix, SOD or SOD and catalase signi®cantly decreased the clastogenic activity, suggesting the involvement of superoxide anion and hydrogen peroxide in the genotoxicity of hydroquinone. However, other species generated in the auto-oxidation process of hydroquinone, such as the semiquinone radical or the quinone, also seem to play a role in its genotoxicity, since the addition of antioxidant enzymes (catalase and SOD) or S9 mix do not lead to a complete abolition of the observed genotoxic activity. These results suggest the existence of at least two mechanisms associated with the genotoxic activity of this compound.

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