Metagenomics/Metabarcoding Sequencing service (16S, 18S, ITS, etc.)

What do we need to know to give you the best price for your project?

• Number of samples
• Amplicon(s) of interest (16S, ITS, etc.), please see below the standard primers we use, if you need other primers, please let us know the exact sequence
• Output per sample
• Do you need Bioinformatics Analysis?

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If you prefer you can contact us by phone through (+351) 926 315 468 (phone call or WhatsApp ) or (+351) 210 438 606

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Our standard conditions

• Quality control of the gDNA, Library preparation and sequencing on Illumina MiSeq (PE300). Bioinformatics Analysis with QIIME 2 if requested.
• Standard output of 50 000-100 000 reads per sample.
• 16S: V3-V4 region, Primer combination
  341F (5’-CCTACGGGNGGCWGCAG-3’)
  785R (5’-GACTACHVGGGTATCTAATCC-3’)
• ITS: ITS1 region, Primer combination
  ITS1f (5’-CTTGGTCATTTAGAGGAAGTAA-3’)
  ITS2 (5’-GCTGCGTTCTTCATCGATGC-3’)

STAB VIDA, LDA.
Madan Parque, Rua dos Inventores, Salas 2.18 e 2.19
2825-182 Caparica - Portugal
Email: sales@stabvida.com
T: +351 210 438 606 | T: +351 926 315 468