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Analiza i genotypowanie fragmentów DNA pozwala na identyfikację organizmów w oparciu o różnice w ich sekwencjach genomowych.  Bada się specyficzne loci w genomie i wykorzystuje  do określenia genotypu danego organizmu. Obecnie najbardziej zaawansowane techniki opierają się na amplifikacji specyficznych polimorficznych regionów w genomie. Metody te mają szerokie zastosowanie wśród różnych dziedzin naukowych m.in.: mapowanie genetyczne zwierząt, filogenetyczne badanie roślin i mikroorganizmów, diagnostyka i medycyna sądowa.

Firma STABVIDA oferuje analizę fragmentów, obejmującą wykrywanie i identyfikację: Polimorfizmu Pojedynczego Nukleotydu (SNP), Polimorfizmu Długości Amplifikowanego Fragmentu i Mikrosatelit. Oferujemy pełny zakres usług bądź elektroforezę kapilarną.Nasi klienci mogą zawsze liczyć na nasze sugestie i porady. Zapraszamy do skorzystania naszych usług!

com levitra, zwany także sekwencjami mikrosatelitarnymi, są to regiony DNA, które zawierają szereg zmiennych, kolejno powtarzających się sekwencji od 2 do 6 par zasad. Regiony te w DNA zostały wybrane jako markery do diagnostyki genetycznej. Ich wykorzystanie wzrosło w takich dziedzinach jak genetyka populacji, analiza korelacji i mapowanie genetyczne.
Mikrosatelity posiadają pożądane cechy: występują w stosunkowo dużej ilości, są regularnie rozmieszczone w poszczególnych regionach genomowego DNA. Ponadto są one niezwykle heterozygotyczne. Firma STAB VIDA oferuje analizę sekwencji mikrosatelitarnych metodą amplifikacji  specyficznych loci (PCR),  z zastosowaniem znakowanych fluorescencyjnie starterów. Następnie produkty reakcji PCR są rozdzielane przy pomocy elektroforezy kapilarnej i identyfikowane fluorescencyjnie. Korzyści wynikające z analizy STR:

1. Mały rozmiar, umożliwiający koamplifikację więcej niż jednego locus w jednej reakcji PCR.
2. Łatwa interpretacja wyników.
3. PCR pozwala na analizę śladowych ilości DNA.
4. Skrócenie czasu analizy.


com levitra, są to zmiany pojedynczej zasady azotowej w DNA. Szacuje się, że ludzki genom zawiera węcej niż 3mln SNP-ów, wspólny dla większości populacji, wśród których 2/3 to substytucja C/T.

Znaczenie SNP-ów:

- niektóre SNP-y flankują mutacje odpowiedzialne za choroby;
- z ewolucyjnego punktu widzenia, SNP są bardzo stabilne, ponieważ nie ulegają dużym zmianom podczas dziedziczenia, więc analiza SNP-ów może pomóc w ustaleniu relacji między grupami ludności.
- uważa się również iż SNP-y związane są z odpowiedzią orgaizmu na niektóre leki. W tym przypadku identyfikacja SNP-ów doprowadzi do poprawy skuteczności terapii w przyszłości.

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Oferujemy wysoko wydajny skrining, w oparciu o najnowsze techniki. Genotypowanie  może  być wykonywane dla poznanych i jeszcze nie zidentyfikowanych mutacji. Będziemy przechowywać Państwa dane tak, aby w każdym momecie istniała możliwość dostarczenia nowej serii próbek do dalszych badań przesiewowych. Próbki mogą być dostarczane w formie oczyszczonego DNA lub innej. Startery mogą być dostarczone przez klienta lub zaprojektowane i syntetyzowane przez STAB VIDA.

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