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¿Cómo pueden los datos de transcriptómica ser usados para estudiar la función de los genes?
La transcriptómica es el análisis de ARN de cualquier especie presente en una célula ya sea ARNm, smallARN o cualquier otra foma  de ARN no codificante. Los datos de transcriptómica proporcionan a los investigadores un buen punto de partida para los estudios funcionales de un nuevo gen. Por ejemplo, si la base de datos de transcriptómica muestra los niveles de expresión de un determinado gen, drásticamente más altos en células cancerígenas en relación a células sanas, significa que este gen podrá desempeñar un papel a nivel de crecimiento celular. O, si un gen se expresa en el tejido adiposo, pero no tejidos óseos o en tejidos musculares, significa que este gen puede llegar a estar implicado en el almacenamiento de lípidos o en el metabolismo.

La secuenciación de pequeños ARNs es una potente aplicación para la secuenciación NGS, permitindo el descubrimiento y la creación de perfiles de microARNs y otros ARNs no codificantes de cualquier organismo, sin la anotación anterior del genoma. Utilizando ARN de bajos insumos, puede generar perfiles de la expresión diferencial de microARNs conocidos, así como detectar nuevas dianas de microARN y una amplia variación en la secuencia o "iso-MIR" adhesiones miRBase. Usando secuenciación de próxima generación, secuenciación de transcriptoma permite detectar e identificar casi todas las clases de moléculas que se transcribe desde el microARN corto para las regiones no traducidas ya 5 'y 3' a lo más largo, mARN. Antes de enviar las muestras por favor verifique:
-Calidad Total RNA: RIN superior a 8,0
-Cantidad total de ARN: 10-20ug. Concentración mínima de 200ng/uL

Vamos a entregar los datos para sus proyectos de:
• ARNm transcripción de análisis de la expresión (de larga duración en ARNm, etiquetas de secuencias expresadas (EST) y ditags),
• Descubrimiento de juevos genes,
• la identificación de genes en los genomas de espacio nuevo,
• inserción-deleción y el descubrimiento de empalme de la variante,
• análisis de los alelos específicos de la expresión y reordenamiento cromosómico.
• Los pequeños ARN de secuenciación;
• El total de la secuencia de ARN:

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