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El método de identificación del sexo en aves a través del ADN se basa en el hecho de que las aves de sexo diferente tienen una composición en sus cromosomas sexuales distinto. Los machos poseen dos cromosomas sexuales idénticos (homogaméticos) y las hembras tienen dos cromosomas sexuales diferentes - el cromosoma Z y el cromosoma W (heterogamético). Durante el proceso laboratorial de identificación del sexo, se beneficia del hecho de que uno de los dos genes presentes en ambos cromosomas (genes CHD) tiene diferente tamaño en el cromosoma Z y en el cromosoma W. Por lo tanto, machos y hembras se identifican fácilmente porque el resultado del análisis que se lleva a cabo es un patrón de bandas en las que los machos poseen una sola banda (que corresponde a la visualización del gen CHD en dos cromosomas idénticos), mientras que las hembras tienen dos bandas (que corresponde a la visualización del gen CHD en el cromosoma Z y en el cromosoma W).

La identificación del sexo de las aves mediante análisis de ADN, realizado a través de muestras de sangre o de plumas, es actualmente el más ventajoso y preferido por los criadores de aves que no presentan dimorfismo sexual (sin diferencias morfológicas visibles entre machos y hembras). Este método tiene las siguientes ventajas:

1. No es necesaria ninguna intervención quirúrgica ni anestesia.
2. Es aplicable a aves de cualquier edad, en cualquier época del año
3. Las aves no están sometidas al estrés de ser transportadas
4. Es un método fiable y aplicable a la mayoría de las especies

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